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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be noticed in many tumors, which ends up in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was identified to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential job in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two additional splice variants had been described but specific nucleotide sequence information and facts just isn't nevertheless out there.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide usually contains the epitope acknowledged because of the antibody.
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This manual outlines further more doable optimization of mobile and immunoassay parameters to make sure the absolute best results are...
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This guide outlines even more achievable optimization of cellular and immunoassay parameters to be sure the very best benefits are...
Antibodies sure to the blocking peptide no more bind into the epitope around the goal protein. By comparing the staining from the blocked antibody as opposed to the antibody on your own, you can see which staining is unique.
Target Details Cyclin E belongs on the hugely conserved cyclin spouse and children, whose users are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which lead for the temporal coordination of each mitotic celebration. Cyclin E varieties a posh with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is also degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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